Sains Malaysiana 52(11)(2023): 3121-3134

http://doi.org/10.17576/jsm-2023-5211-08

Probiotics Potential of Lactic Acid Bacteria from Horse Milk in Tambora, West Nusa Tenggara, Indonesia

(Potensi Probiotik Bakteria Asid Laktik daripada Susu Kuda di Tambora, Barat Nusa Tenggara, Indonesia)

SULISTIANI^1,2, R. HARYO BIMO SETIARTO^1,2,*, FERA R DEWI^1,2, DANDY YUSUF^1,2, AHMAD FATHONI^1,2, RINI HANDAYANI¹, LUTFI ANGGADHANIA¹, LUTFI ANSHORY¹, NINU SETIANINGRUM¹ & TATIK KHUSNIATI¹

¹Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Jalan Raya Jakarta-Bogor Km 46, Soekarno Science Centre, Cibinong, Bogor, 16911 West Java, Indonesia

²Research Collaboration Center for Traditional Fermentation, National Research and Innovation Agency (BRIN) Indonesia

Received: 22 November 2022/Accepted: 25 October 2023

Abstract

This study explores the probiotic potential of lactic acid bacteria (LAB) in horse milk from Tambora, West Nusa Tenggara by examining its functional and probiotic attributes, including bile salt tolerance, low pH, simulated gastric juice (SGJ), simulated intestinal juice (SIJ), antibacterial activity, as well as bile salt hydrolase (BSH) genes. Genotyping of LAB was performed using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) analysis and then identified based on the 16S rRNA gene. A total of 25 LAB isolates showed the ability to grow at low pH, tolerant to bile, and survived under SIJ and SGJ conditions. The BSH gene was confirmed in three isolates, namely: SK1-28, SK2-30, and SK2-34. Results of RFLP-PCR analysis showed that the LAB isolates were grouped into three groups based on the number and molecular weight of the differences DNA fragments. The 16S rRNA analysis showed that the first two groups were Lacticaseibacillus rhamnosus, whereas the third group was Lactiplantibacillus plantarum. In addition, all Lacticaseibacillus rhamnosusisolates in group I showed the ability to grow at pH 9.0, but not group II. It can be concluded that Lacticaseibacillus rhamnosus and Lactiplantibacillus plantarum can be used as the indigenous probiotic bacteria source from Indonesia.

Keywords: Horse's milk; lactic acid bacteria (LAB); Lacticaseibacillus rhamnosus; Lactiplantibacillus plantarum; probiotics

Abstrak

Penyelidikan ini mengkaji potensi probiotik bakteria asid laktik (LAB) dalam susu kuda dari Tambora, Barat Nusa Tenggara dengan meneliti sifat fungsian dan probiotiknya, termasuk toleransi garam hempedu, pH rendah, jus gastrik disimulasi (SGJ), jus usus disimulasi (SIJ) aktiviti antibakteria serta gen hidrolase garam hempedu (BSH). Genotip LAB dilakukan menggunakan analisis tindak balas rantai polimorfisme-polimerase sekatan panjang serpihan (RFLP-PCR) dan kemudian dikenal pasti berdasarkan gen rRNA 16S. Sebanyak 25 pencilan LAB menunjukkan keupayaan untuk membesar pada pH rendah, bertoleransi dengan hempedu dan bermandiri dalam keadaan SIJ dan SGJ. Gen BSH telah disahkan dalam tiga pencilan iaitu: SK1-28, SK2-30 dan SK2-34. Keputusan analisis RFLP-PCR menunjukkan bahawa pencilan LAB dikelompokkan kepada tiga kumpulan berdasarkan bilangan dan berat molekul perbezaan serpihan DNA. Analisis rRNA 16S menunjukkan bahawa dua kumpulan pertama ialah Lacticaseibacillus rhamnosus, manakala kumpulan ketiga ialah Lactiplantibacillus plantarum. Di samping itu, semua pencilan Lacticaseibacillus rhamnosus dalam kumpulan I menunjukkan keupayaan untuk berkembang pada pH 9.0 tetapi bukan kumpulan II. Dapat disimpulkan bahawa Lacticaseibacillus rhamnosus dan Lactiplantibacillus plantarum boleh digunakan sebagai sumber bakteria probiotik asli dari Indonesia.

Kata kunci: Bakteria asid laktik (LAB); Lacticaseibacillus rhamnosus; Lactiplantibacillus plantarum; probiotik; susu kuda

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*Corresponding author; email: haryobimo88@gmail.com